Comprehensive RNA profiling of villous trophoblast and decidua basalis in pregnancies complicated by preterm birth following intra-amniotic infection
Introduction We performed RNA sequencing with the primary goal of discovering key
placental villous trophoblast (VT) and decidua basalis (DB) transcripts differentially
expressed in intra-amniotic infection (IAI)-induced preterm birth (PTB). Methods RNA was
extracted from 15 paired VT and DB specimens delivered of women with: 1) spontaneous
PTB in the setting of amniocentesis-proven IAI and histological chorioamnionitis (n= 5); 2)
spontaneous idiopathic PTB (iPTB, n= 5); and 3) physiologic term pregnancy (n= 5). RNA …
placental villous trophoblast (VT) and decidua basalis (DB) transcripts differentially
expressed in intra-amniotic infection (IAI)-induced preterm birth (PTB). Methods RNA was
extracted from 15 paired VT and DB specimens delivered of women with: 1) spontaneous
PTB in the setting of amniocentesis-proven IAI and histological chorioamnionitis (n= 5); 2)
spontaneous idiopathic PTB (iPTB, n= 5); and 3) physiologic term pregnancy (n= 5). RNA …