[PDF][PDF] Pancreas-specific Cre driver lines and considerations for their prudent use

MA Magnuson, AB Osipovich - Cell metabolism, 2013 - cell.com
Cell metabolism, 2013cell.com
Cre/LoxP has broad utility for studying the function, development, and oncogenic
transformation of pancreatic cells in mice. Here we provide an overview of the Cre driver
lines that are available for such studies. We discuss how variegated expression, transgene
silencing, and recombination in undesired cell types have conspired to limit the performance
of these lines, sometimes leading to serious experimental concerns. We also discuss
preferred strategies for achieving high-fidelity driver lines and remind investigators of the …
Cre/LoxP has broad utility for studying the function, development, and oncogenic transformation of pancreatic cells in mice. Here we provide an overview of the Cre driver lines that are available for such studies. We discuss how variegated expression, transgene silencing, and recombination in undesired cell types have conspired to limit the performance of these lines, sometimes leading to serious experimental concerns. We also discuss preferred strategies for achieving high-fidelity driver lines and remind investigators of the continuing need for caution when interpreting results obtained from any Cre/LoxP-based experiment performed in mice.
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