Quantitative analysis of phospholipids by HPLC with a light scattering evaporating detector–application to raw materials for cosmetic use
I Becart, C Chevalier, JP Biesse - Journal of High Resolution …, 1990 - Wiley Online Library
I Becart, C Chevalier, JP Biesse
Journal of High Resolution Chromatography, 1990•Wiley Online LibraryA method is described for separation and quantitative analysis of various phospholipids and
sphingolipids by high performance liquid chromatography on a silica gel column with a
mobile phase consisting of chloroform, methanol, and ammonium hydroxide. Gradient
elution is necessary. A study of the light scattering evaporative detector response is
presented. The method has been applied to the analysis of phospholipids of a commercial
soja lecithin and a pig brain extract.
sphingolipids by high performance liquid chromatography on a silica gel column with a
mobile phase consisting of chloroform, methanol, and ammonium hydroxide. Gradient
elution is necessary. A study of the light scattering evaporative detector response is
presented. The method has been applied to the analysis of phospholipids of a commercial
soja lecithin and a pig brain extract.
Abstract
A method is described for separation and quantitative analysis of various phospholipids and sphingolipids by high performance liquid chromatography on a silica gel column with a mobile phase consisting of chloroform, methanol, and ammonium hydroxide. Gradient elution is necessary. A study of the light scattering evaporative detector response is presented. The method has been applied to the analysis of phospholipids of a commercial soja lecithin and a pig brain extract.
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