[HTML][HTML] Blood sphingolipidomics in healthy humans: impact of sample collection methodology
SM Hammad, JS Pierce, F Soodavar, KJ Smith… - Journal of lipid …, 2010 - ASBMB
We used a HPLC-MS/MS methodology for determination of a basic metabolomic profile (18:
1, 18: 0 sphingoid backbone, C 14-C 26 N-acyl part) of" normal" sphingolipid levels in
human serum and plasma. Blood was collected from healthy males and nonpregnant
females under fasting and nonfasting conditions with and without anticoagulants.
Sphingolipids analyzed included sphingoid bases, sphingosine and dihydrosphingosine,
their 1-phosphates (S1P and dhS1P), molecular species (C n-) of ceramide (Cer) …
1, 18: 0 sphingoid backbone, C 14-C 26 N-acyl part) of" normal" sphingolipid levels in
human serum and plasma. Blood was collected from healthy males and nonpregnant
females under fasting and nonfasting conditions with and without anticoagulants.
Sphingolipids analyzed included sphingoid bases, sphingosine and dihydrosphingosine,
their 1-phosphates (S1P and dhS1P), molecular species (C n-) of ceramide (Cer) …