Role of calcineurin in angiotensin II-induced cardiac myocyte hypertrophy of rats.
M Fu, J Zhang, S Xu, Y Pang, N Liu… - … = Chung-kuo i Hsueh k'o …, 2001 - europepmc.org
M Fu, J Zhang, S Xu, Y Pang, N Liu, C Tang
Chinese Medical Sciences Journal= Chung-kuo i Hsueh k'o Hsueh tsa Chih, 2001•europepmc.orgObjective The present study investigated the role of calcineurin in angiotensin II (AngII)-
induced cardiac myocyte hypertrophy of rats. Method The primary cardiac myocytes were
cultured under the standard conditions. The calcineurin activity in AngII-treated
cardiomyocytes was tested by using PNPP; protein synethsis rate was assessed by 3H-
leucine incorporation; atrial natriuretic factor (ANF) mRNA level was determined by Northern
blot analysis. Cell viability was estimated by lactate dehydrogenase (LDH) levels in cultured …
induced cardiac myocyte hypertrophy of rats. Method The primary cardiac myocytes were
cultured under the standard conditions. The calcineurin activity in AngII-treated
cardiomyocytes was tested by using PNPP; protein synethsis rate was assessed by 3H-
leucine incorporation; atrial natriuretic factor (ANF) mRNA level was determined by Northern
blot analysis. Cell viability was estimated by lactate dehydrogenase (LDH) levels in cultured …
Objective
The present study investigated the role of calcineurin in angiotensin II (AngII)-induced cardiac myocyte hypertrophy of rats.
Method
The primary cardiac myocytes were cultured under the standard conditions. The calcineurin activity in AngII-treated cardiomyocytes was tested by using PNPP; protein synethsis rate was assessed by 3H-leucine incorporation; atrial natriuretic factor (ANF) mRNA level was determined by Northern blot analysis. Cell viability was estimated by lactate dehydrogenase (LDH) levels in cultured medium and by dyed cell numbers.
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