Evaluation of a New Tool for Exploring Podocyte Biology: Mouse: Nphs1: 5′ Flanking Region Drives LacZ Expression in Podocytes
MJ Moeller, IA Kovari, LB Holzman - Journal of the American …, 2000 - journals.lww.com
Study of podocyte biology has been hampered by limitations in available experimental
models that both recapitulate the in vivo phenotypes of this cell and can be readily and
specifically manipulated at the molecular level. Transgenic manipulation of the podocyte
represents one approach that might circumvent these limitations. The purpose of this study
was to identify a promoter-enhancer that would direct the expression of transgenes in a
podocyte-specific manner. The nephrin (Nphs1) promoter was considered a good candidate …
models that both recapitulate the in vivo phenotypes of this cell and can be readily and
specifically manipulated at the molecular level. Transgenic manipulation of the podocyte
represents one approach that might circumvent these limitations. The purpose of this study
was to identify a promoter-enhancer that would direct the expression of transgenes in a
podocyte-specific manner. The nephrin (Nphs1) promoter was considered a good candidate …