[PDF][PDF] Endothelial dysfunction is correlated with microalbuminuria in children with short-duration type 1 diabetes

LFF Adan, AMT Ladeia, CL Frota, L Pinho, E Stefanelli - 2005 - repositorio.ufba.br
LFF Adan, AMT Ladeia, CL Frota, L Pinho, E Stefanelli
2005repositorio.ufba.br
METHODS—This cross-sectional study included 18 randomly selected type 1 diabetic
patients (13 boys) followed up at a public health assistance center, with disease duration of
2.9 1.2 years (0.7–4.9 years) and without clinical evidence of vascular complications, and 14
healthy control subjects (7 boys) without chronic diseases or hypercholesterolemia, matched
by age (2 years) and BMI. Patients with retinopathy, hypertension, obesity, thyroid disease,
and history of smoking were excluded. Laboratory data included fasting blood glucose …
METHODS—This cross-sectional study included 18 randomly selected type 1 diabetic patients (13 boys) followed up at a public health assistance center, with disease duration of 2.9 1.2 years (0.7–4.9 years) and without clinical evidence of vascular complications, and 14 healthy control subjects (7 boys) without chronic diseases or hypercholesterolemia, matched by age (2 years) and BMI. Patients with retinopathy, hypertension, obesity, thyroid disease, and history of smoking were excluded. Laboratory data included fasting blood glucose, plasma lipids, HbA1c (A1C), creatinine, TSH, and microalbuminuria.
After the subjects had been lying in supine position for 10 min in a stabletemperature room, the response to reactive hyperemia (FMD) was evaluated. The diameter of the brachial artery was measured in a longitudinal section (2–15 cm above the elbow) with a high-resolution vascular ultrasound. Reactive hyperemia was induced by occluding arterial blood by using a sphygmomanometer inflated to 100 mmHg above the systolic pressure. After 4 min, the cuff was released. The arterial flow velocity was measured by a pulsed Doppler signal at a 60-degree angle to the vessel during the resting scan and for 15 s after the cuff deflated. The artery was scanned for 30 s before and 90 s after cuff release. An experienced vascular ultrasonographer, blind to the patient’s diagnosis, performed and analyzed all images recorded in a high-quality computer. An eletrocardiogram was recorded with the ultrasound images. Diameter changes were derived as percentage changes relative to the first scan. Baseline blood flow was estimated by multiplying the flow velocity integral by heart rate and the square of the radius of the artery. Reactive hyperemia was calculated as the maximum flow measured during the first
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