[CITATION][C] Antigen presentation by normal B cells, B cell tumors, and macrophages: functional and biochemical comparison.
RW Chesnut, SM Colon, HM Grey - Journal of immunology …, 1982 - journals.aai.org
RW Chesnut, SM Colon, HM Grey
Journal of immunology (Baltimore, Md.: 1950), 1982•journals.aai.orgMATERIALS AND METHODS Mice. BDF, and BALB/c mice were bred at the National Jewish
Hospital and were approximately 10 wk of age when used. Antigens. Keyhole limpet
hemocyanin (KLH) was obtained from Calbiochem. La Jolla. CA and LPS from E. coli was
purchased from Difco. Detroit, MI. NRGG was prepared from the serum of New Zealand
White rabbits by DE-52 (Pharmacia, Piscataway, NJ) column chromatography. The IgG
fraction of RAMlG was similarly prepared by using the serum of rabbits immunized with …
Hospital and were approximately 10 wk of age when used. Antigens. Keyhole limpet
hemocyanin (KLH) was obtained from Calbiochem. La Jolla. CA and LPS from E. coli was
purchased from Difco. Detroit, MI. NRGG was prepared from the serum of New Zealand
White rabbits by DE-52 (Pharmacia, Piscataway, NJ) column chromatography. The IgG
fraction of RAMlG was similarly prepared by using the serum of rabbits immunized with …
MATERIALS AND METHODS
Mice. BDF, and BALB/c mice were bred at the National Jewish Hospital and were approximately 10 wk of age when used. Antigens. Keyhole limpet hemocyanin (KLH) was obtained from Calbiochem. La Jolla. CA and LPS from E. coli was purchased from Difco. Detroit, MI. NRGG was prepared from the serum of New Zealand White rabbits by DE-52 (Pharmacia, Piscataway, NJ) column chromatography. The IgG fraction of RAMlG was similarly prepared by using the serum of rabbits immunized with multiple subcutaneous injections of the mouse myeloma protein PC-5,(e. IgG2a) in complete adjuvant (H-37 Ra, Difco).
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