Skin punch biopsy explant culture for derivation of primary human fibroblasts

M Vangipuram, D Ting, S Kim, R Diaz… - JoVE (Journal of …, 2013 - jove.com
Tissues and cell lines derived from an individual with disease are ideal sources to study
disease-related cellular phenotypes. Patient-derived fibroblasts in this protocol have been
successfully used in the derivation of induced pluripotent stem cells to model disease1.
Early passages of these fibroblasts can also be used for cell-based functional assays to
study specific disease pathways, mechanisms2 and subsequent drug screening
approaches. The advantage of the presented protocol over enzymatic procedures are 1) the …