Growing axons develop a highly motile structure at their tip, termed the growth cone. The
growth cone contacts extracellular environmental cues to navigate axonal growth. Netrin,
slit, semaphorin, and ephrins are known guidance molecules that can attract or repel axons
upon binding to receptors and co-receptors on the axon. The activated receptors initiate
various signaling molecules in the growth cone that alter the structure and movement of the
neuron. Here, we describe the detailed protocol for a stripe assay to assess the ability of a …