Measuring influenza neuraminidase inhibition antibody titers by enzyme-linked lectin assay
J Gao, L Couzens, MC Eichelberger - JoVE (Journal of Visualized …, 2016 - jove.com
Antibodies to neuraminidase (NA), the second most abundant surface protein on influenza
virus, contribute toward protection against influenza. Traditional methods to measure NA
inhibiting (NI) antibody titers are not practical for routine serology. This protocol describes
the enzyme-linked lectin assay (ELLA), a practical alternative method to measure NI titers
that is performed in 96 well plates coated with a large glycoprotein substrate, fetuin. NA
cleaves terminal sialic acids from fetuin, exposing the penultimate sugar, galactose. Peanut …
virus, contribute toward protection against influenza. Traditional methods to measure NA
inhibiting (NI) antibody titers are not practical for routine serology. This protocol describes
the enzyme-linked lectin assay (ELLA), a practical alternative method to measure NI titers
that is performed in 96 well plates coated with a large glycoprotein substrate, fetuin. NA
cleaves terminal sialic acids from fetuin, exposing the penultimate sugar, galactose. Peanut …
