Macrophages are often considered as a reservoir of latent infection in HIV+ patients, and
their infection may indeed be very important functionally. However, some quantitative
studies did not find high infection frequencies in peripheral blood monocytes. Since
lymphoid organs are the major site of infection, macrophage infection was tested in spleens
removed from HIV+ patients for treatment of different syndromes. Ten replicates of limiting
dilutions from different cell populations were submitted to a nested PCR specific to …