Integration of human immunodeficiency virus type 1 in untreated infection occurs preferentially within genes

H Liu, EC Dow, R Arora, JT Kimata, LM Bull… - Journal of …, 2006 - Am Soc Microbiol
H Liu, EC Dow, R Arora, JT Kimata, LM Bull, RC Arduino, AP Rice
Journal of virology, 2006Am Soc Microbiol
Previous analyses of human immunodeficiency virus type 1 (HIV-1) integration sites
generated in infections in vitro or in patients in whom viral replication was repressed by
antiviral therapy have demonstrated a preference for integration within protein-coding
genes. We analyzed integration sites in peripheral blood mononuclear cells (PBMCs),
spleen, lymph node, and cerebral cortex from patients with untreated HIV-1 infections. The
great majority of integration sites in each tissue were within genes. Statistical analyses of the …
Abstract
Previous analyses of human immunodeficiency virus type 1 (HIV-1) integration sites generated in infections in vitro or in patients in whom viral replication was repressed by antiviral therapy have demonstrated a preference for integration within protein-coding genes. We analyzed integration sites in peripheral blood mononuclear cells (PBMCs), spleen, lymph node, and cerebral cortex from patients with untreated HIV-1 infections. The great majority of integration sites in each tissue were within genes. Statistical analyses of the frequencies of integration in genes in PBMCs and lymph tissue demonstrated a strong preference for integration within genes. Although the sample size for brain tissue was too small to demonstrate a clear statistical preference for integration in genes, four of the five integration sites identified in brain were within genes. Taken together, our data indicate that HIV-1 preferentially integrates within genes during untreated infection.
American Society for Microbiology