Novel method for the rapid isolation of RPE cells specifically for RNA extraction and analysis
CXZ Wang, K Zhang, B Aredo, H Lu… - Experimental eye …, 2012 - Elsevier
RPE cells are involved in the pathogenesis of many retinal diseases. Accurate analysis of
RPE gene expression profiles in different scenarios will increase our understanding of
disease mechanisms. Our objective in this study was to develop an improved method for the
isolation of RPE cells, specifically for RNA analysis. Mouse RPE cells were isolated using
different techniques, including mechanical dissociation techniques and a new technique we
refer to here as “Simultaneous RPE cell Isolation and RNA Stabilization”(SRIRS method) …
RPE gene expression profiles in different scenarios will increase our understanding of
disease mechanisms. Our objective in this study was to develop an improved method for the
isolation of RPE cells, specifically for RNA analysis. Mouse RPE cells were isolated using
different techniques, including mechanical dissociation techniques and a new technique we
refer to here as “Simultaneous RPE cell Isolation and RNA Stabilization”(SRIRS method) …