Use of direct current electroretinography for analysis of retinal pigment epithelium function in mouse models

M Yu, NS Peachey - Mouse Retinal Phenotyping: Methods and Protocols, 2018 - Springer
Mouse Retinal Phenotyping: Methods and Protocols, 2018Springer
A monolayer of pigmented epithelial cells, the retinal pigment epithelium (RPE), supports
photoreceptor function in many ways. Consistent with these roles, RPE dysfunction
underlies a number of hereditary retinal disorders. To monitor RPE function in vivo models
for these conditions, we adapted an electroretinographic (ERG) technique based on direct
current amplification (DC-ERG). This chapter describes the main features of this approach
and its application to mouse models involving the RPE.
Abstract
A monolayer of pigmented epithelial cells, the retinal pigment epithelium (RPE), supports photoreceptor function in many ways. Consistent with these roles, RPE dysfunction underlies a number of hereditary retinal disorders. To monitor RPE function in vivo models for these conditions, we adapted an electroretinographic (ERG) technique based on direct current amplification (DC-ERG). This chapter describes the main features of this approach and its application to mouse models involving the RPE.
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