An efficient inducible RPE-Selective cre transgenic mouse line
M Chen, L Kim, CW Lu, H Zeng, D Vollrath - Experimental eye research, 2021 - Elsevier
Cre-mediated modulation of gene function in the murine retinal pigment epithelium (RPE)
has been widely used, but current postnatal RPE-selective Cre driver lines suffer from
limited recombination efficiency and/or ectopic or mosaic expression. We sought to generate
a transgenic mouse line with consistently efficient RPE-selective Cre activity that could be
temporally regulated. We used ϕC31 integrase to insert a DNA construct encoding a human
BEST1 promoter fragment driving a Cre recombinase estrogen receptor fusion (BEST1 …
has been widely used, but current postnatal RPE-selective Cre driver lines suffer from
limited recombination efficiency and/or ectopic or mosaic expression. We sought to generate
a transgenic mouse line with consistently efficient RPE-selective Cre activity that could be
temporally regulated. We used ϕC31 integrase to insert a DNA construct encoding a human
BEST1 promoter fragment driving a Cre recombinase estrogen receptor fusion (BEST1 …