Conditional control of gene expression in the mouse
M Lewandoski - Nature Reviews Genetics, 2001 - nature.com
Nature Reviews Genetics, 2001•nature.com
One of the most powerful tools that the molecular biology revolution has given us is the
ability to turn genes on and off at our discretion. In the mouse, this has been accomplished
by using binary systems in which gene expression is dependent on the interaction of two
components, resulting in either transcriptional transactivation or DNA recombination. During
recent years, these systems have been used to analyse complex and multi-staged biological
processes, such as embryogenesis and cancer, with unprecedented precision. Here, I …
ability to turn genes on and off at our discretion. In the mouse, this has been accomplished
by using binary systems in which gene expression is dependent on the interaction of two
components, resulting in either transcriptional transactivation or DNA recombination. During
recent years, these systems have been used to analyse complex and multi-staged biological
processes, such as embryogenesis and cancer, with unprecedented precision. Here, I …
Abstract
One of the most powerful tools that the molecular biology revolution has given us is the ability to turn genes on and off at our discretion. In the mouse, this has been accomplished by using binary systems in which gene expression is dependent on the interaction of two components, resulting in either transcriptional transactivation or DNA recombination. During recent years, these systems have been used to analyse complex and multi-staged biological processes, such as embryogenesis and cancer, with unprecedented precision. Here, I review these systems and discuss certain studies that exemplify the advantages and limitations of each system.
nature.com