Dual RMCE for efficient re-engineering of mouse mutant alleles

M Osterwalder, A Galli, B Rosen, WC Skarnes… - Nature …, 2010 - nature.com
Nature methods, 2010nature.com
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently
re-engineer the thousands of available conditional alleles in mouse embryonic stem cells.
dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional
alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags,
reporters and mutant coding regions into an endogenous locus of interest in an easy and
highly efficient manner.
Abstract
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.
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