[HTML][HTML] Vascular endothelial growth factor stimulates prostacyclin production and activation of cytosolic phospholipase A2 in endothelial cells via p42/p44 mitogen …

C Wheeler-Jones, R Abu-Ghazaleh, R Cospedal… - FEBS letters, 1997 - Elsevier
C Wheeler-Jones, R Abu-Ghazaleh, R Cospedal, RA Houliston, J Martin, I Zachary
FEBS letters, 1997Elsevier
Vascular endothelial growth factor (VEGF) stimulated a time-and concentration-dependent
increase in PGI2 synthesis in human umbilical vein endothelial cells with a mean maximum
increase of 2-fold above basal levels at 25 ng/ml after 60 min. VEGF also rapidly stimulated
the release of arachidonic acid and phosphorylation and activation of cytosolic
phospholipase A2 (cPLA2). The VEGF-related factor, placenta growth factor (PlGF), had little
effect on PGI2 synthesis, arachidonic acid release or cPLA2 activation. PD98059, a selective …
Vascular endothelial growth factor (VEGF) stimulated a time- and concentration-dependent increase in PGI2 synthesis in human umbilical vein endothelial cells with a mean maximum increase of 2-fold above basal levels at 25 ng/ml after 60 min. VEGF also rapidly stimulated the release of arachidonic acid and phosphorylation and activation of cytosolic phospholipase A2 (cPLA2). The VEGF-related factor, placenta growth factor (PlGF), had little effect on PGI2 synthesis, arachidonic acid release or cPLA2 activation. PD98059, a selective inhibitor of MAP kinase kinase, caused complete inhibition of VEGF-stimulated MAP kinase activity, PGI2 synthesis and cPLA2 gel retardation, but had no effect on VEGF-induced vWF secretion. These findings provide the first evidence that VEGF can stimulate PGI2 synthesis via cPLA2-mediated arachidonic acid release and indicate that VEGF stimulation of this biosynthetic pathway may occur, at least in part, via activation of p42/p44 MAP kinases.
Elsevier