Molecular analysis of HLA class I and class II antigen loss mutants reveals a homozygous deletion of the DR, DQ, and part of the DP region: implications for class II …
H Erlich, JS Lee, JW Petersen, T Bugawan… - Human Immunology, 1986 - Elsevier
The mutant human B-lymphoblastoid cell lines, 721.174 and 721.180, previously reported to
exhibit greatly reduced expression of human HLA class I and II antigens (DeMars et al., Hum
Immunol 11: 77, 1984), were analyzed by Southern blotting using class II cDNA and
genomic clones as hybridization probes. All genomic sequences complementary to DRα,
DRß, DQα, and DQß probes were absent from these mutants. DZα genomic sequences were
deleted as were the DPα1 and DPß2 loci but the DPß2 and most, if not all, of the DPα2 locus …
exhibit greatly reduced expression of human HLA class I and II antigens (DeMars et al., Hum
Immunol 11: 77, 1984), were analyzed by Southern blotting using class II cDNA and
genomic clones as hybridization probes. All genomic sequences complementary to DRα,
DRß, DQα, and DQß probes were absent from these mutants. DZα genomic sequences were
deleted as were the DPα1 and DPß2 loci but the DPß2 and most, if not all, of the DPα2 locus …