Techniques for the observation and measurement of red blood cell velocity in vessels of the guinea pig cochlea
AL Nuttall - Hearing research, 1987 - Elsevier
Fluorescence techniques combined with intravital microscopy provide a powerful approach
to the study of cochlear blood microcirculation. In the current study, fluorescein
isothiocyanate conjugated to high molecular weight dextrans was added to plasma to
enhance the visual contrast of flowing blood in microscopic images from the guinea pig
cochlea. Photometric signals, obtained from video pictures of the blood vessels, provided a
means to continuously measure red cell velocity by using crosscorrelation algorithms to …
to the study of cochlear blood microcirculation. In the current study, fluorescein
isothiocyanate conjugated to high molecular weight dextrans was added to plasma to
enhance the visual contrast of flowing blood in microscopic images from the guinea pig
cochlea. Photometric signals, obtained from video pictures of the blood vessels, provided a
means to continuously measure red cell velocity by using crosscorrelation algorithms to …