Establishing primary adult fibroblast cultures from rodents

A Seluanov, A Vaidya, V Gorbunova - JoVE (Journal of Visualized …, 2010 - jove.com
The importance of using primary cells, rather than cancer cell lines, for biological studies is
becoming widely recognized. Primary cells are preferred in studies of cell cycle control,
apoptosis, and DNA repair, as cancer cells carry mutations in genes involved in these
processes. Primary cells cannot be cultured indefinitely due to the onset of replicative
senescence or aneuploidization. Hence, new cultures need to be established regularly. The
procedure for isolation of rodent embryonic fibroblasts is well established, but isolating adult …