Molecular methods for evaluation of virological status of nonhuman primates challenged with simian immunodeficiency or simian-human immunodeficiency viruses
EM Lee, HK Chung, J Livesay, J Suschak… - Journal of virological …, 2010 - Elsevier
Nonhuman primates represent a robust model to evaluate preclinical efficacy of HIV-1
vaccine and therapeutic strategies. Plasma and tissue viral RNA as well as tissue proviral
DNA load are key parameters in assessing efficacy of vaccines and therapeutics against
simian immunodeficiency virus (SIV) or simian-human immunodeficiency virus (SHIV)
challenge. To quantitate SIV RNA in plasma and tissues, an isothermal nucleic acid
sequence-based amplification (NASBA) method using real-time detection of amplified RNA …
vaccine and therapeutic strategies. Plasma and tissue viral RNA as well as tissue proviral
DNA load are key parameters in assessing efficacy of vaccines and therapeutics against
simian immunodeficiency virus (SIV) or simian-human immunodeficiency virus (SHIV)
challenge. To quantitate SIV RNA in plasma and tissues, an isothermal nucleic acid
sequence-based amplification (NASBA) method using real-time detection of amplified RNA …