Paramyxovirus infections in ex vivo lung slice cultures of different host species
DT Nguyen, RD de Vries, M Ludlow… - Journal of Virological …, 2013 - Elsevier
Paramyxoviruses, including measles virus (MV), human metapneumovirus (HMPV), human
respiratory syncytial virus (HRSV) and canine distemper virus (CDV), are transmitted via the
respiratory route. Despite their close phylogenetic relationship, the pathogenesis of these
viruses is very different. To study viral tropism and replication ex vivo, a protocol for the
inflation of lungs with low-melting-point agarose mixed with culture medium was
established. Lung slices were prepared and remained viable in culture at 37° C for at least 7 …
respiratory syncytial virus (HRSV) and canine distemper virus (CDV), are transmitted via the
respiratory route. Despite their close phylogenetic relationship, the pathogenesis of these
viruses is very different. To study viral tropism and replication ex vivo, a protocol for the
inflation of lungs with low-melting-point agarose mixed with culture medium was
established. Lung slices were prepared and remained viable in culture at 37° C for at least 7 …
