Extracellular Ig domains 1 and 2 of Robo are important for ligand (Slit) binding
Z Liu, K Patel, H Schmidt, W Andrews, A Pini… - Molecular and Cellular …, 2004 - Elsevier
Robo, the receptor for the midline repellent Slit, is a member of the cell adhesion molecule
(CAM) Ig superfamily. We have recently demonstrated that members of the Robo family
(Robo1 and Robo2) interact homophilically and heterophilically, thereby functioning to
promote neurite outgrowth. Here, we describe a series of in vitro experiments to dissect the
Robo ligand-interacting domains by deleting specific extracellular regions of the Robo1
molecule, generating a series of mutant proteins. Using these, we demonstrate that Ig …
(CAM) Ig superfamily. We have recently demonstrated that members of the Robo family
(Robo1 and Robo2) interact homophilically and heterophilically, thereby functioning to
promote neurite outgrowth. Here, we describe a series of in vitro experiments to dissect the
Robo ligand-interacting domains by deleting specific extracellular regions of the Robo1
molecule, generating a series of mutant proteins. Using these, we demonstrate that Ig …