Development of IL-22–producing NK lineage cells from umbilical cord blood hematopoietic stem cells in the absence of secondary lymphoid tissue
Blood, The Journal of the American Society of Hematology, 2011•ashpublications.org
Human secondary lymphoid tissues (SLTs) contain interleukin-22 (IL-22)–producing cells
with an immature NK phenotype. Given their location, these cells are difficult to study. We
have generated large numbers of NK22 cells from hematopoietic stem cells. HSC-derived
NK22 cells show a CD56+ CD117highCD94− phenotype, consistent with stage III NK
progenitors. Like freshly isolated SLT stage III cells, HSC-derived NK22 cells express
NKp44, CD161, CCR6, IL1 receptor, AHR, and ROR-γτ. IL-1β and IL-23 stimulation results …
with an immature NK phenotype. Given their location, these cells are difficult to study. We
have generated large numbers of NK22 cells from hematopoietic stem cells. HSC-derived
NK22 cells show a CD56+ CD117highCD94− phenotype, consistent with stage III NK
progenitors. Like freshly isolated SLT stage III cells, HSC-derived NK22 cells express
NKp44, CD161, CCR6, IL1 receptor, AHR, and ROR-γτ. IL-1β and IL-23 stimulation results …
Abstract
Human secondary lymphoid tissues (SLTs) contain interleukin-22 (IL-22)–producing cells with an immature NK phenotype. Given their location, these cells are difficult to study. We have generated large numbers of NK22 cells from hematopoietic stem cells. HSC-derived NK22 cells show a CD56+CD117highCD94− phenotype, consistent with stage III NK progenitors. Like freshly isolated SLT stage III cells, HSC-derived NK22 cells express NKp44, CD161, CCR6, IL1 receptor, AHR, and ROR-γτ. IL-1β and IL-23 stimulation results in significant IL-22 but not interferon-γ production. Supernatant from these cells increases CD54 expression on mesenchymal stem cells. Thus, IL-22–producing NK cells can be generated in the absence of SLT. HSC-derived NK22 cells will be valuable in understanding this rare NK subset and create the opportunity for human translational clinical trials.
ashpublications.org