Direct evidence of lipid translocation between adipocytes and prostate cancer cells with imaging FTIR microspectroscopy
E Gazi, P Gardner, NP Lockyer, CA Hart, MD Brown… - Journal of lipid …, 2007 - jlr.org
Various epidemiological studies show a positive correlation between high intake of dietary
FAs and metastatic prostate cancer (CaP). Moreover, CaP metastasizes to the bone marrow,
which harbors a rich source of lipids stored within adipocytes. Here, we use Fourier
transform infrared (FTIR) microspectroscopy to study adipocyte biochemistry and to
demonstrate that PC-3 cells uptake isotopically labeled FA [deuterated palmitic acid (D 31-
PA)] from an adipocyte. Using this vibrational spectroscopic technique, we detected …
FAs and metastatic prostate cancer (CaP). Moreover, CaP metastasizes to the bone marrow,
which harbors a rich source of lipids stored within adipocytes. Here, we use Fourier
transform infrared (FTIR) microspectroscopy to study adipocyte biochemistry and to
demonstrate that PC-3 cells uptake isotopically labeled FA [deuterated palmitic acid (D 31-
PA)] from an adipocyte. Using this vibrational spectroscopic technique, we detected …
