[HTML][HTML] Characterization of tissue-specific differential DNA methylation suggests distinct modes of positive and negative gene expression regulation
Background DNA methylation plays an important role in regulating gene expression during
many biological processes. However, the mechanism of DNA-methylation-dependent gene
regulation is not fully understood. Here, we explore two possible DNA methylation regulatory
mechanisms with opposite modes of gene expression regulation. Results By comparing the
genome-wide methylation and expression patterns in different tissues, we find that majority
of tissue-specific differentially methylated regions (T-DMRs) are negatively correlated with …
many biological processes. However, the mechanism of DNA-methylation-dependent gene
regulation is not fully understood. Here, we explore two possible DNA methylation regulatory
mechanisms with opposite modes of gene expression regulation. Results By comparing the
genome-wide methylation and expression patterns in different tissues, we find that majority
of tissue-specific differentially methylated regions (T-DMRs) are negatively correlated with …
Background
DNA methylation plays an important role in regulating gene expression during many biological processes. However, the mechanism of DNA-methylation-dependent gene regulation is not fully understood. Here, we explore two possible DNA methylation regulatory mechanisms with opposite modes of gene expression regulation.
Results
By comparing the genome-wide methylation and expression patterns in different tissues, we find that majority of tissue-specific differentially methylated regions (T-DMRs) are negatively correlated with expression of their associated genes (negative T-DMRs), consistent with the classical dogma that DNA methylation suppresses gene expression; however, a significant portion of T-DMRs are positively correlated with gene expression (positive T-DMRs). We observe that the positive T-DMRs have similar genomic location as negative T-DMRs, except that the positive T-DMRs are more enriched in the promoter regions. Both positive and negative T-DMRs are enriched in DNase I hypersensitivity sites (DHSs), suggesting that both are likely to be functional. The CpG sites of both positive and negative T-DMRs are also more evolutionarily conserved than the genomic background. Interestingly, the putative target genes of the positive T-DMR are enriched for negative regulators such as transcriptional repressors, suggesting a novel mode of indirect DNA methylation inhibition of expression through transcriptional repressors. Likewise, two distinct sets of DNA sequence motifs exist for positive and negative T-DMRs, suggesting that two distinct sets of transcription factors (TFs) are involved in positive and negative regulation mediated by DNA methylation.
Conclusions
We find both negative and positive association between T-DMRs and gene expression, which implies the existence of two different mechanisms of DNA methylation-dependent gene regulation.
Springer