c-Fos immunoreactivity in the brain after esophageal acid stimulation
The goal of this study was to use c-Fos immunohistochemistry to establish a rat model for
studying the central projection of the esophageal afferent neurons during acid exposure. A
cannula was placed in the esophagus of anesthetized Wistar rats with the tip approximately
2 cm above the lower esophageal sphincter (LES). Hydrochloric acid (0.1 N HCl, 50 mmol/L)
with pepsin (3,200–4,500 U/mL), at pH 1.6, was then perfused into the esophagi of the
experimental rats (n= 8) at 10 mL/hr continuously for 50 minutes. Normal saline solution …
studying the central projection of the esophageal afferent neurons during acid exposure. A
cannula was placed in the esophagus of anesthetized Wistar rats with the tip approximately
2 cm above the lower esophageal sphincter (LES). Hydrochloric acid (0.1 N HCl, 50 mmol/L)
with pepsin (3,200–4,500 U/mL), at pH 1.6, was then perfused into the esophagi of the
experimental rats (n= 8) at 10 mL/hr continuously for 50 minutes. Normal saline solution …