Targeting and tracing antigens in live cells with fluorescent nanobodies
U Rothbauer, K Zolghadr, S Tillib, D Nowak… - Nature …, 2006 - nature.com
Nature methods, 2006•nature.com
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp.
with fluorescent proteins to generate fluorescent, antigen-binding nanobodies
(chromobodies) that can be expressed in living cells. We demonstrate that chromobodies
can recognize and trace antigens in different subcellular compartments throughout S phase
and mitosis. Chromobodies should enable new functional studies, as potentially any
antigenic structure can be targeted and traced in living cells in this fashion.
with fluorescent proteins to generate fluorescent, antigen-binding nanobodies
(chromobodies) that can be expressed in living cells. We demonstrate that chromobodies
can recognize and trace antigens in different subcellular compartments throughout S phase
and mitosis. Chromobodies should enable new functional studies, as potentially any
antigenic structure can be targeted and traced in living cells in this fashion.
Abstract
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
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