TNFα-induced up-regulation of miR-155 inhibits adipogenesis by down-regulating early adipogenic transcription factors

S Liu, Y Yang, J Wu - Biochemical and biophysical research …, 2011 - Elsevier
S Liu, Y Yang, J Wu
Biochemical and biophysical research communications, 2011Elsevier
Tumor necrosis factor α (TNFα) is known to inhibit adipogenesis, but the molecular
mechanism of this inhibition remains elusive. In the present study, we found that TNFα-
induced inhibition of adipogenesis mainly occurs when 3T3-L1 preadipocytes are treated
with TNFα within 2h induction of adipogenesis. We revealed that TNFα treatment results in
the up-regulation of miR-155 through the NFκB pathway in 3T3-L1 cells. This
overexpression of miR-155 may suppress the expression of C/EBPβ and CREB by directly …
Tumor necrosis factor α (TNFα) is known to inhibit adipogenesis, but the molecular mechanism of this inhibition remains elusive. In the present study, we found that TNFα-induced inhibition of adipogenesis mainly occurs when 3T3-L1 preadipocytes are treated with TNFα within 2h induction of adipogenesis. We revealed that TNFα treatment results in the up-regulation of miR-155 through the NFκB pathway in 3T3-L1 cells. This overexpression of miR-155 may suppress the expression of C/EBPβ and CREB by directly targeting their 3′ untranslated regions (3′ UTRs). Importantly, anti-miR-155 reduces the TNFα-induced inhibition of adipogenesis, whereas exogenous expression of mir-155 inhibits adipogenesis. Taken together, these findings reveal a novel role for TNFα in the regulation of anti-adipogenic miRNAs.
Elsevier