The Fc receptor on thymus-derived lymphocytes. I. Detection of a subpopulation of murine T lymphocytes bearing the Fc receptor.
RD Stout, LA Herzenberg - The Journal of experimental medicine, 1975 - rupress.org
RD Stout, LA Herzenberg
The Journal of experimental medicine, 1975•rupress.orgUtilizing the fluorescence-activated cell sorter (FACS) and washed murine antibody-antigen
complexes formed in antibody excess, we have demonstrated the presence of the Fc
receptor on the surface of a distinct subpopulation of murine T lymphocytes. No differences
in intensity of labeling with the complexes was observed when the Fc+ T lymphocytes were
compared with Fc+ B lymphocytes. The majority of Fc+ T lymphocytes are small lymphocytes
determined by light-scattering characteristics on the FACS. Separating Fc+ from Fc-T …
complexes formed in antibody excess, we have demonstrated the presence of the Fc
receptor on the surface of a distinct subpopulation of murine T lymphocytes. No differences
in intensity of labeling with the complexes was observed when the Fc+ T lymphocytes were
compared with Fc+ B lymphocytes. The majority of Fc+ T lymphocytes are small lymphocytes
determined by light-scattering characteristics on the FACS. Separating Fc+ from Fc-T …
Utilizing the fluorescence-activated cell sorter (FACS) and washed murine antibody-antigen complexes formed in antibody excess, we have demonstrated the presence of the Fc receptor on the surface of a distinct subpopulation of murine T lymphocytes. No differences in intensity of labeling with the complexes was observed when the Fc+ T lymphocytes were compared with Fc+ B lymphocytes. The majority of Fc+ T lymphocytes are small lymphocytes determined by light-scattering characteristics on the FACS. Separating Fc+ from Fc- T lymphocytes from spleens of mice primed 1 wk or 1 mo previously with keyhole limpet hemocyanin (KLH) revealed that the T cells capable of cooperating with DNP-KLH primed B cells to give an adoptive anti-DNP PFC response do not bear the Fc receptor.
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