Myeloid derived suppressor cells are numerically, functionally and phenotypically different in patients with multiple myeloma

J Favaloro, T Liyadipitiya, R Brown, S Yang… - Leukemia & …, 2014 - Taylor & Francis
J Favaloro, T Liyadipitiya, R Brown, S Yang, H Suen, N Woodland, N Nassif, D Hart…
Leukemia & lymphoma, 2014Taylor & Francis
Myeloid derived suppressor cells (MDSCs) are a heterogeneous population of cells that
have been implicated as inhibitors of lymphopoiesis in patients with malignancies. They
have a consensus phenotype of CD33+/CD11b+/HLA-DRlo/− and can be further divided
into CD15+ granulocytic (G-MDSC) and CD14+ monocytic (M-MDSC) subsets. We
characterized MDSCs in patients with multiple myeloma (MM) and found a significant
increase in G-MDSCs in the blood of patients with progressive MM. Flow-sorted MDSCs …
Abstract
Myeloid derived suppressor cells (MDSCs) are a heterogeneous population of cells that have been implicated as inhibitors of lymphopoiesis in patients with malignancies. They have a consensus phenotype of CD33+/CD11b+/HLA-DRlo/− and can be further divided into CD15 + granulocytic (G-MDSC) and CD14 + monocytic (M-MDSC) subsets. We characterized MDSCs in patients with multiple myeloma (MM) and found a significant increase in G-MDSCs in the blood of patients with progressive MM. Flow-sorted MDSCs from patients with MM induced the generation of regulatory T cells (Treg). MDSCs from both patients with MM and aged-matched controls demonstrated a dose-dependent inhibition of lymphocyte proliferation in carboxyfluorescein succinimidyl ester (CFSE)-tracking experiments. Granulocyte colony stimulating factor (G-CSF) administered to induce stem cell mobilization caused an increase in the number of MDSCs in the peripheral blood of patients with MM and a concentration of these immune-suppressive cells in peripheral blood stem cell collections. MDSCs are likely to cause immune dysfunction in patients with MM.
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