Immunohistochemistry is highly sensitive and specific for the detection of V600E BRAF mutation in melanoma
GV Long, JS Wilmott, D Capper… - The American journal …, 2013 - journals.lww.com
This study investigated the sensitivity and specificity of immunohistochemical (IHC) analysis
using an anti-BRAF antibody to detect the presence of the BRAF V600E mutation in patients
with metastatic melanoma. A total of 100 patients with American Joint Committee on Cancer
stage IIIC unresectable or stage IV melanoma and who underwent tumor DNA BRAF
mutation testing were selected. Paraffin-embedded, formalin-fixed melanoma biopsies were
analyzed for the BRAF mutation status by independent, blinded observers using both …
using an anti-BRAF antibody to detect the presence of the BRAF V600E mutation in patients
with metastatic melanoma. A total of 100 patients with American Joint Committee on Cancer
stage IIIC unresectable or stage IV melanoma and who underwent tumor DNA BRAF
mutation testing were selected. Paraffin-embedded, formalin-fixed melanoma biopsies were
analyzed for the BRAF mutation status by independent, blinded observers using both …