Heme oxygenase-1 enhances autophagy in podocytes as a protective mechanism against high glucose-induced apoptosis

C Dong, H Zheng, S Huang, N You, J Xu, X Ye… - Experimental cell …, 2015 - Elsevier
C Dong, H Zheng, S Huang, N You, J Xu, X Ye, Q Zhu, Y Feng, Q You, H Miao, D Ding, Y Lu
Experimental cell research, 2015Elsevier
Injury and loss of podocytes play vital roles in diabetic nephropathy progression. Emerging
evidence suggests autophagy, which is induced by multiple stressors including
hyperglycemia, plays a protective role. Meanwhile, heme oxygenase-1 (HO-1) possesses
powerful anti-apoptotic properties. Therefore, we investigated the impact of autophagy on
podocyte apoptosis under diabetic conditions and its association with HO-1. Mouse
podocytes were cultured in vitro; apoptosis was detected by flow cytometry. Transmission …
Abstract
Injury and loss of podocytes play vital roles in diabetic nephropathy progression. Emerging evidence suggests autophagy, which is induced by multiple stressors including hyperglycemia, plays a protective role. Meanwhile, heme oxygenase-1 (HO-1) possesses powerful anti-apoptotic properties. Therefore, we investigated the impact of autophagy on podocyte apoptosis under diabetic conditions and its association with HO-1. Mouse podocytes were cultured in vitro; apoptosis was detected by flow cytometry. Transmission electron microscopy and biochemical autophagic flux assays were used to measure the autophagy markers microtubule-associated protein 1 light chain 3-II (LC3-II) and beclin-1. LC3-II and beclin-1 expression peaked 12–24 h after exposing podocytes to high glucose. Inhibition of autophagy with 3-methyladenine or Beclin-1 siRNAs or Atg 5 siRNAs sensitized cells to apoptosis, suggesting autophagy is a survival mechanism. HO-1 inactivation inhibited autophagy, which aggravated podocyte injury in vitro. Hemin-induced autophagy also protected podocytes from hyperglycemia in vitro and was abrogated by HO-1 siRNA. Adenosine monophosphate-activated protein kinase phosphorylation was higher in hemin-treated and lower in HO-1 siRNA-treated podocytes. Suppression of AMPK activity reversed HO-1-mediated Beclin-1 upregulation and autophagy, indicating HO-1-mediated autophagy is AMPK dependent. These findings suggest HO-1 induction and regulation of autophagy are potential therapeutic targets for diabetic nephropathy.
Elsevier