Loss of T cell responses following long-term cryopreservation
RE Owen, E Sinclair, B Emu, JW Heitman… - Journal of …, 2007 - Elsevier
Although cryopreservation of peripheral blood mononuclear cells (PBMC) is a commonly
used technique, the degree to which it affects subsequent functional studies has not been
well defined. Here we demonstrate that long-term cryopreservation has detrimental effects
on T cell IFN-γ responses in human immunodeficiency virus (HIV) infected individuals. Long-
term cryopreservation caused marked decreases in CD4+ T cell responses to whole
proteins (HIV p55 and cytomegalovirus (CMV) lysate) and HIV peptides, and more limited …
used technique, the degree to which it affects subsequent functional studies has not been
well defined. Here we demonstrate that long-term cryopreservation has detrimental effects
on T cell IFN-γ responses in human immunodeficiency virus (HIV) infected individuals. Long-
term cryopreservation caused marked decreases in CD4+ T cell responses to whole
proteins (HIV p55 and cytomegalovirus (CMV) lysate) and HIV peptides, and more limited …
