The role of miR-190a-5p contributes to diabetic neuropathic pain via targeting SLC17A6
D Yang, Q Yang, X Wei, Y Liu, D Ma, J Li… - Journal of pain …, 2017 - Taylor & Francis
D Yang, Q Yang, X Wei, Y Liu, D Ma, J Li, Y Wan, Y Luo
Journal of pain research, 2017•Taylor & FrancisIntroduction MicroRNAs play a key role in neuropathic pain. In a previous study, miR-190a-
5p was significantly downregulated in diabetic neuropathic pain (DNP). However, the role
and pathological mechanism of miR-190a-5p in DNP still remain unclear. Materials and
methods DNP model was established. The paw withdrawal thresholds were measured to
assess the mechanical nociceptive response. Dual-luciferase reporter assay was used to
confirm the target gene of microRNA. The expressions of microRNA, gene, and protein were …
5p was significantly downregulated in diabetic neuropathic pain (DNP). However, the role
and pathological mechanism of miR-190a-5p in DNP still remain unclear. Materials and
methods DNP model was established. The paw withdrawal thresholds were measured to
assess the mechanical nociceptive response. Dual-luciferase reporter assay was used to
confirm the target gene of microRNA. The expressions of microRNA, gene, and protein were …
Introduction
MicroRNAs play a key role in neuropathic pain. In a previous study, miR-190a-5p was significantly downregulated in diabetic neuropathic pain (DNP). However, the role and pathological mechanism of miR-190a-5p in DNP still remain unclear.
Materials and methods
DNP model was established. The paw withdrawal thresholds were measured to assess the mechanical nociceptive response. Dual-luciferase reporter assay was used to confirm the target gene of microRNA. The expressions of microRNA, gene, and protein were detected by the quantitative real-time polymerase chain reaction or Western blot. The levels of IL-1β and IL-6 were detected with the enzyme-linked immuno sorbent assay.
Results
Compared with the control sample, the expression of miR-190a-5p was decreased and SLC17A6 was increased in the spinal tissue from those developing DNP. The bioinformatics and luciferase reporter assay demonstrated that SLC17A6 is a direct target of miR-190a-5p. Up-regulation of miR-190a-5p and inhibition of SLC17A6 could significantly weaken the painful behavior and reduce IL-1β and IL-6 level in DNP.
Conclusion
miR-190a-5p is involved in DNP via targeting SLC17A6, and miR-190a-5p and SLC17A6 may be the therapeutic targets of this disease.
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