β-Adrenergic signaling plays an important role in the natural history of dilated cardiomyopathies. Chronic activation of β-adrenergic receptors (β₁-AR and β₂-AR) during periods of cardiac stress ultimately harms the failing heart by mechanisms that include alterations in gene expression. Here, we show that stimulation of β-ARs with isoproterenol in neonate rat ventricular myocytes causes a “fetal” response in the relative activities of the human cardiac fetal and/or adult gene promoters that includes repression of the human and rat α-myosin heavy chain (α-MyHC) promoters with simultaneous activation of the human atrial natriuretic peptide (ANP) and rat β-MyHC promoters. We also show that the promoter changes correlate with changes in endogenous gene expression as measured by mRNA expression. Furthermore, we show that these changes are specifically mediated by the β₁-AR, but not the β₂-AR, and are independent of α₁-AR stimulation. We also demonstrate that the fetal gene response is independent of cAMP and protein kinase A, whereas inhibition of Ca²⁺/calmodulin-dependent protein kinase (CaMK) pathway blocks isoproterenol-mediated fetal gene program induction. Finally, we show that induction of the fetal program is dependent on activation of the L-type Ca²⁺ channel. We conclude that in neonatal rat cardiac myocytes, agonist-occupied β₁-AR mobilizes Ca²⁺ stores to activate fetal gene induction through cAMP independent pathways that involve CaMK.