Expression of cytokine genes, including IL-6, in human malignant melanoma cell lines
MP Colombo, C Maccalli, S Mattei, C Melani… - Melanoma …, 1992 - journals.lww.com
As a preliminary to transducing human melanoma cells with lymphokine genes, we sought
for constitutive gene expression and production of eight interleukins, tumour necrosis factors
and granulocytecolony stimulating factor in 19 human melanoma cell lines. Conversion of
RNA into cDNA by reverse transcriptase and polymerase chain reaction (RT-PCR) were
employed to evaluate gene expression while enzyme-linked immunosorbent assays (ELISA)
or biological assays were used to assess the presence of proteins. No expression of …
for constitutive gene expression and production of eight interleukins, tumour necrosis factors
and granulocytecolony stimulating factor in 19 human melanoma cell lines. Conversion of
RNA into cDNA by reverse transcriptase and polymerase chain reaction (RT-PCR) were
employed to evaluate gene expression while enzyme-linked immunosorbent assays (ELISA)
or biological assays were used to assess the presence of proteins. No expression of …