[HTML][HTML] Involvement of valosin-containing protein, an ATPase co-purified with IκBα and 26 S proteasome, in ubiquitin-proteasome-mediated degradation of IκBα

RM Dai, E Chen, DL Longo, CM Gorbea… - Journal of Biological …, 1998 - ASBMB
The inactivation of the prototype NF-κB inhibitor, IκBα, occurs through a series of ordered
processes including phosphorylation, ubiquitin conjugation, and proteasome-mediated
degradation. We identify valosin-containing protein (VCP), an AAA (ATPases associated
with a variety of cellular activities) family member, that co-precipitates with IκBα immune
complexes. The ubiquitinated IκBα conjugates readily associate with VCP both in vivo and in
vitro, and this complex appears dissociated from NF-κB. In ultracentrifugation analysis …