Paraneoplastic β cell dedifferentiation in nondiabetic patients with pancreatic cancer

Y Wang, Q Ni, J Sun, M Xu, J Xie… - The Journal of …, 2020 - academic.oup.com
Y Wang, Q Ni, J Sun, M Xu, J Xie, J Zhang, Y Fang, G Ning, Q Wang
The Journal of Clinical Endocrinology & Metabolism, 2020academic.oup.com
Context Beta-cell dedifferentiation was recently proposed as a mechanism of β-cell
dysfunction, but whether it can be a trigger of β-cell failure preceding hyperglycemia in
humans is uncertain. Pancreatic cancer can cause new-onset diabetes, yet the underlying
mechanism is unknown. Objective To investigate whether β-cell dedifferentiation is present
in nondiabetic pancreatic ductal adenocarcinoma (PDAC) patients, we examined pancreatic
islets from 15 nondiabetic patients with benign tumors (control) and 15 nondiabetic PDAC …
Context
Beta-cell dedifferentiation was recently proposed as a mechanism of β-cell dysfunction, but whether it can be a trigger of β-cell failure preceding hyperglycemia in humans is uncertain. Pancreatic cancer can cause new-onset diabetes, yet the underlying mechanism is unknown.
Objective
To investigate whether β-cell dedifferentiation is present in nondiabetic pancreatic ductal adenocarcinoma (PDAC) patients, we examined pancreatic islets from 15 nondiabetic patients with benign tumors (control) and 15 nondiabetic PDAC patients.
Design
We calculated the number of hormone-negative endocrine cells and evaluated important markers of β-cell dedifferentiation and function in the paraneoplastic islets. We assessed tumor-related inflammatory changes under the pancreatic cancer microenvironment and their influence on β-cell identity.
Results
We found nearly 10% of nonhormone expressing endocrine cells in nondiabetic PDAC subjects. The PDAC islets were dysfunctional, evidenced by low expression of Glucose transporter 2 (GLUT2) and Urocortin3 (UCN3), and concomitant upregulation of Aldehyde Dehydrogenase 1 Family Member A3 (ALDH1A3) expression and proinsulin accumulation. Pancreatic cancer caused paraneoplastic inflammation with enhanced tissue fibrosis, monocytes/macrophages infiltration, and elevated inflammatory cytokines. Moreover, we detected β-cell dedifferentiation and defects in GSIS in islets exposed to PANC-1 (a cell line established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male)-conditioned medium. In a larger cohort, we showed high prevalence of new-onset diabetes in PDAC subjects, and fasting blood glucose (FBG) was found to be an additional useful parameter for early diagnosis of PDAC.
Conclusions
Our data provide a rationale for β-cell dedifferentiation in the pathogenesis of pancreatic cancer–associated diabetes. We propose that β-cell dedifferentiation can be a trigger for β-cell failure in humans, before hyperglycemia occurs.
Oxford University Press