Separation of plasma lipoproteins by density-gradient ultracentrifugation
TG Redgrave, DCK Roberts, CE West - Analytical biochemistry, 1975 - Elsevier
TG Redgrave, DCK Roberts, CE West
Analytical biochemistry, 1975•ElsevierThe separation of plasma lipoproteins into VLDL, LDL, and HDL by a single 24-hr
ultracentrifugation in swinging-bucket rotors is described. The mean recovery of loaded
lipoprotein cholesterol is 89.3±1.3%. The method employs a discontinuous salt gradient and
separates VLDL, LDL, and HDL as verified by cellulose acetate-and immuno-
electrophoresis. The method offers some advantages for research applications.
ultracentrifugation in swinging-bucket rotors is described. The mean recovery of loaded
lipoprotein cholesterol is 89.3±1.3%. The method employs a discontinuous salt gradient and
separates VLDL, LDL, and HDL as verified by cellulose acetate-and immuno-
electrophoresis. The method offers some advantages for research applications.
The separation of plasma lipoproteins into VLDL, LDL, and HDL by a single 24-hr ultracentrifugation in swinging-bucket rotors is described. The mean recovery of loaded lipoprotein cholesterol is 89.3 ± 1.3%. The method employs a discontinuous salt gradient and separates VLDL, LDL, and HDL as verified by cellulose acetate- and immuno-electrophoresis. The method offers some advantages for research applications.
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