Mitochondria assemble into flexible networks. Here we present a simple method for the
simultaneous quantification of mitochondrial membrane potential and network morphology
that is based on computational co-localisation analysis of differentially imported fluorescent
marker proteins. Established in, but not restricted to, Saccharomyces cerevisiae, MitoLoc
reproducibly measures changes in membrane potential induced by the uncoupling agent
CCCP, by oxidative stress, in respiratory deficient cells, and in∆ fzo1,∆ ref2, and∆ dnm1 …