[HTML][HTML] The BUME method: A novel automated chloroform-free 96-well total lipid extraction method for blood plasma [S]

L Löfgren, M Ståhlman, GB Forsberg, S Saarinen… - Journal of lipid …, 2012 - ASBMB
Lipid extraction from biological samples is a critical and often tedious preanalytical step in
lipid research. Primarily on the basis of automation criteria, we have developed the BUME
method, a novel chloroform-free total lipid extraction method for blood plasma compatible
with standard 96-well robots. In only 60 min, 96 samples can be automatically extracted with
lipid profiles of commonly analyzed lipid classes almost identically and with absolute
recoveries similar or better to what is obtained using the chloroform-based reference …