Next-generation biomarkers based on 100-parameter functional super-resolution microscopy TIS
W Schubert, A Gieseler, A Krusche, P Serocka… - New biotechnology, 2012 - Elsevier
Functional super-resolution (fSR) microscopy is based on the automated toponome imaging
system (TIS). fSR-TIS provides insight into the myriad of different cellular functionalities by
direct imaging of large subcellular protein networks in morphologically intact cells and
tissues, referred to as the toponome. By cyclical fluorescence imaging of at least 100
molecular cell components, fSR-TIS overcomes the spectral limitations of fluorescence
microscopy, which is the essential condition for the detection of protein network structures in …
system (TIS). fSR-TIS provides insight into the myriad of different cellular functionalities by
direct imaging of large subcellular protein networks in morphologically intact cells and
tissues, referred to as the toponome. By cyclical fluorescence imaging of at least 100
molecular cell components, fSR-TIS overcomes the spectral limitations of fluorescence
microscopy, which is the essential condition for the detection of protein network structures in …