[HTML][HTML] Humanized SFTPA1 and SFTPA2 transgenic mice reveal functional divergence of SP-A1 and SP-A2: formation of tubular myelin in vivo requires both gene …
Surfactant protein A (SP-A) plays a role in lung innate immunity and surfactant-related
functions. Two functional genes, SP-A1 (SFTPA1) and SP-A2 (SFTPA2), are present in
humans and primates (rodents have one gene). Single gene SP-A1 or SP-A2 proteins
expressed in vitro are functional. To study their role in vivo, we generated humanized
transgenic (hTG) C57BL/6 mice, SP-A1 (6A 4) and SP-A2 (1A 3). The SP-A cDNA in
experimental constructs was driven by the 3.7-kb SP-C promoter. Positive hTG mice were …
functions. Two functional genes, SP-A1 (SFTPA1) and SP-A2 (SFTPA2), are present in
humans and primates (rodents have one gene). Single gene SP-A1 or SP-A2 proteins
expressed in vitro are functional. To study their role in vivo, we generated humanized
transgenic (hTG) C57BL/6 mice, SP-A1 (6A 4) and SP-A2 (1A 3). The SP-A cDNA in
experimental constructs was driven by the 3.7-kb SP-C promoter. Positive hTG mice were …