Response differences between human CD4+ and CD8+ T-cells during CD28 costimulation: implications for immune cell-based therapies and studies related to the …
I Laux, A Khoshnan, C Tindell, D Bae, X Zhu… - Clinical …, 2000 - Elsevier
Since CD28 costimulation is critical for T-cell activation, there is great interest in CD28 as a
target for immuntherapeutic approaches. We show that stimulation of human CD4+ and
CD8+ T-cells differs in their responsiveness to stimulation with anti-CD3/CD28-coated
beads, as surrogate antigen-presenting cells. While the CD4+ subset responded with
sustained proliferation, CD8+ T-cells grew for a limited period only and failed to produce IL-2
beyond the first few days in culture. This decrease is accompanied with an increased rate of …
target for immuntherapeutic approaches. We show that stimulation of human CD4+ and
CD8+ T-cells differs in their responsiveness to stimulation with anti-CD3/CD28-coated
beads, as surrogate antigen-presenting cells. While the CD4+ subset responded with
sustained proliferation, CD8+ T-cells grew for a limited period only and failed to produce IL-2
beyond the first few days in culture. This decrease is accompanied with an increased rate of …
