[HTML][HTML] Antibody uptake into neurons occurs primarily via clathrin-dependent Fcγ receptor endocytosis and is a prerequisite for acute tau protein clearance
EE Congdon, J Gu, HBR Sait, EM Sigurdsson - Journal of Biological …, 2013 - ASBMB
Tau immunotherapy is effective in transgenic mice, but the mechanisms of Tau clearance
are not well known. To this end, Tau antibody uptake was analyzed in brain slice cultures
and primary neurons. Internalization was rapid (< 1 h), saturable, and substantial compared
with control mouse IgG. Furthermore, temperature reduction to 4° C, an excess of unlabeled
mouse IgG, or an excess of Tau antibodies reduced uptake in slices by 63, 41, and 62%,
respectively (p= 0.002, 0.04, and 0.005). Uptake strongly correlated with total and insoluble …
are not well known. To this end, Tau antibody uptake was analyzed in brain slice cultures
and primary neurons. Internalization was rapid (< 1 h), saturable, and substantial compared
with control mouse IgG. Furthermore, temperature reduction to 4° C, an excess of unlabeled
mouse IgG, or an excess of Tau antibodies reduced uptake in slices by 63, 41, and 62%,
respectively (p= 0.002, 0.04, and 0.005). Uptake strongly correlated with total and insoluble …
