[HTML][HTML] A high-throughput, bead-based, antigen-specific assay to assess the ability of antibodies to induce complement activation
S Fischinger, JK Fallon, AR Michell, T Broge… - Journal of …, 2019 - Elsevier
The complement system plays a critical role in innate immune defense against pathogens,
both via non-specific direct pathogen recognition and killing or via antigen-specific indirect
recruitment by complement fixing antibodies. While various assays for measuring
complement activation have been developed, few provide a high-throughput, sample-
sparing approach to interrogate the qualitative differences in the ability of antibodies to drive
complement activation. Here we present a high-throughput, sample-sparing, bead-based …
both via non-specific direct pathogen recognition and killing or via antigen-specific indirect
recruitment by complement fixing antibodies. While various assays for measuring
complement activation have been developed, few provide a high-throughput, sample-
sparing approach to interrogate the qualitative differences in the ability of antibodies to drive
complement activation. Here we present a high-throughput, sample-sparing, bead-based …