[PDF][PDF] Deciphering hematopoietic stem cells in their niches: a critical appraisal of genetic models, lineage tracing, and imaging strategies

C Joseph, JM Quach, CR Walkley, SW Lane, CL Celso… - Cell stem cell, 2013 - cell.com
Cell stem cell, 2013cell.com
In recent years, technical developments in mouse genetics and imaging equipment have
substantially advanced our understanding of hematopoietic stem cells (HSCs) and their
niche. The availability of numerous Cre strains for targeting HSCs and microenvironmental
cells provides extensive flexibility in experimental design, but it can also pose significant
challenges due to strain-specific differences in cell specificity. Here we outline various
genetic approaches for isolating, detecting, and ablating HSCs and niche components and …
In recent years, technical developments in mouse genetics and imaging equipment have substantially advanced our understanding of hematopoietic stem cells (HSCs) and their niche. The availability of numerous Cre strains for targeting HSCs and microenvironmental cells provides extensive flexibility in experimental design, but it can also pose significant challenges due to strain-specific differences in cell specificity. Here we outline various genetic approaches for isolating, detecting, and ablating HSCs and niche components and provide a guide for advantages and caveats to consider. We also discuss opportunities and limitations presented by imaging technologies that allow investigation of HSC behavior in situ.
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